What is TB-500?

TB-500 is a laboratory-synthesized peptide modeled after Thymosin Beta-4, a naturally occurring peptide found in nearly all human and animal cells. It belongs to a family of 16 highly conserved peptides that play a central role in cellular function and tissue repair. One of its most studied properties is its strong interaction with actin—the structural protein critical for cell shape, movement, and division. TB-500 regulates actin availability by preventing polymerization and functioning as an actin-sequestering peptide within eukaryotic cells.

Scientific studies identified TB-500 as a gene upregulated during the early phases of blood vessel formation, where it supports the sprouting of new blood vessels from existing ones. It has been detected in wound fluid, and when administered in research models, it has demonstrated the ability to accelerate wound closure, support muscle regeneration, and shorten recovery time following tissue injury. A key mechanism underlying these effects is its ability to enhance cellular migration, particularly in endothelial cells and keratinocytes, which are vital to tissue growth and repair. TB-500 also influences the production of enzymes that remodel the extracellular matrix, further contributing to healing and tissue adaptation.

Unlike many growth factors, TB-500 displays unique wound-healing properties. It does not bind strongly to extracellular matrix proteins and, because of its relatively small molecular weight, can diffuse across tissues efficiently. Its role in actin regulation is particularly noteworthy, as actin can comprise up to 10% of the total protein content of a cell, making it fundamental to cellular architecture and genetic activity.

TB-500 Peptide Structure

• Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser

• Molecular Formula: C212H350N56O78S

• Molecular Weight: 4963.44 g/mol

• CAS Number: 77591-33-4

• PubChem CID: 16132341

• Synonyms: Thymosin Beta-4

Research Highlights of TB-500

Preclinical and laboratory studies investigating TB-500 (Thymosin Beta-4) suggest multiple areas of interest, including:

• Supporting endothelial cell differentiation (blood vessel development)

• Stimulating angiogenesis (formation of new blood vessels from existing ones)

• Enhancing keratinocyte migration for tissue repair

• Promoting collagen synthesis and deposition

• Demonstrating anti-inflammatory activity within joint and connective tissues

• Encouraging muscle growth and improved endurance and strength in experimental settings

• Relaxing muscle spasms and enhancing muscle tone

• Facilitating intercellular exchange of nutrients and signaling molecules

• Accelerating tissue regeneration in response to injury

• Improving flexibility of connective tissue by reducing stiffness

• Preventing the development of fibrous adhesions and scar tissue in ligaments, tendons, and muscle fibers

Disclaimer

All TB-500 provided by 1stPharma is intended strictly for laboratory-based and in-vitro research purposes. This compound is not a medicine, drug, or supplement, and has not been reviewed or approved by the FDA or any regulatory authority for medical use. It must not be administered to humans or animals under any circumstances.